| IL-3

Interleukin 3 (IL-3) is a protein that is encoded by the IL3 gene localized on chromosome 5q31.1. IL-3 is produced as a monomer by activated T cells, monocytes/macrophages and stroma cells.The major function of IL-3 cytokine is to regulate the concentrations of various blood-cell types.It induces proliferation and differentiation in both early pluripotent stem cells and committed progenitors.It also has many more specific effects like the regeneration of platelets and potentially aids in early antibody isotype switching.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	100	50	25	125	6.25	3.12	1.56	0
OD	2.47	1.73	1.33	0.92	0.63	0.44	0.32	0.09
Corrected OD	2.38	1.64	1.24	0.83	0.54	0.35	0.23	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat IL-3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	82-101	96
Plasma(n=8)	93-105	102
Cell Culture Supernates(n=8)	97-108	105

 

| Linearity

Samples were spiked with high concentrations of Rat IL-3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-96	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-105	87-108	104-115
	Average (%)	94	98	111